Analysis Note

ControlSpinal Cord, brain membrane lysate

Application

Research Sub CategoryNeuroinflammation & Pain

Research CategoryNeuroscience

Immunohistochemistry:
A previous lot was used at 1:100-1:1,000 dilution.

Western Blot Analysis:
A previous lot was used at 1:500 dilution.

Optimal working dilutions must be determined by the end user.

Anti-Substance P Receptor Antibody, pain detects level of Substance P Receptor & has been published & validated for use in IF, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Substance P is a tachykinin family neuropeptide that functions as a neurotransmitter and neuromodulator. In the CNS, Substance P is involved in the regulation of mood, anxiety, sleep, saiety, reinforcement, respiratory rhythm and pain processing. In nociception, the neuropeptide is released at local spinal cord synapses. Recent research now shows substance P is potentially involved in endothelial cell differentiation as well as diverse roles in neuroinflammatory response. The endogenous receptor for Substance P is neurokinin 1 receptor (NK1 receptor), which belongs to the tachykinin receptor sub-family of GPCRs.

Immunogen

Synthetic peptide that corresponds to a 23 amino acid sequence (385-407) of the COOH terminus of the rat Substance P Receptor (NK-1).

Epitope: C terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB5800

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal serum containing no preservative.

Unpurified

Quality

Evaluated by western blot on Mouse brain membrane lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected NK-1R on 10 µg of Mouse brain membrane lysates.

Specificity

Specific for the Substance P Receptor (NK-1).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

35 kDa